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'Disruption of mitochondrial function in pancreatic beta-cells' - ANAT 396 Undergraduate Research Project Application Form

INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").

INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted here. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature"). Ask your supervisor to sign her/his section near the bottom. Take it to the department* corresponding to the course number in Section A; this may or may not be your own department. (* EXCEPTIONS: For NSCI 396 and COGS 396, please bring it to the Interdisciplinary Programs Adviser in Dawson Hall.) Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.

INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and send a copy of this form (with signatures) to the Office for Undergraduate Research in Science (either fax, or internal mail to Dawson Hall 408-A, or PDF scan + email).

QUESTIONS OR FEEDBACK? Contact the Office for Undergraduate Research in Science.

Supervisor's Name: Jennifer Estall / Craig Mandato

Supervisor's Email: jennifer [dot] estall [at] ircm [dot] qc [dot] ca

Supervisor's Phone: 514-987-5688

Supervisor's Website: www.ircm.qc.ca/estall

Supervisor's department: Anatomy and Cell Biology

Course number: ANAT 396 (Anatomy and Cell Biology)

Term: Summer 2013

Project start date: Wednesday, May 1st, 2013

Project end date: Friday, August 30th, 2013

Project title: Disruption of mitochondrial function in pancreatic beta-cells

Project description: Mitochondrial dysfunction is thought to be contributing factor to the growing epidemic of diabetes. Our lab specializes in transcriptional regulation of mitochondrial function and recently created a new mouse model to investigate how pre-existing defects in mitochondrial metabolism impact beta-cell biology and the pathogenesis of diabetes. Using the cre/LoxP system, we generated a beta-cell specific knock-out of a key transcriptional regulator of mitochondrial biogenesis and function. We have characterized the metabolic phenotype of these mice and find they have significant alterations in glucose homeostasis, beta-cell survival, and insulin secretion in vivo.

The project involves investigating how our genetic knock-out model specifically impacts beta-cell survival and function in vitro. Specifically, the applicant will help to determine whether mitochondrial mass and function is decreased in immortalized and primary cell cultures using fluorescent mitochondrial markers and quantitative microscopy. This will involve working with animal models, culture of cells, treatment of cells, and quantification of mass and function using state-of-the-art scanning microscopy.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance (40%), participation in lab meetings (journal club) (10%), and a final written research report (50%), all evaluated and graded by the supervisor.

Project status: This project is taken. The professor has no more '396' projects this term.

How students can apply: N/A; this project is filled.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Animal subjects; Handling chemicals; Using lasers.

Student's Name:

Student's McGill ID:

Student's McGill Email:

Student's Phone:

Student's Program:

Student's Level (U0 / U1 / U2 / U3):

Student's signature - I certify that this course is with a different supervisor and on a different topic than any previous '396' course I have taken. I have not applied for another '396' course in this term:

Supervisor's signature - I give my permission for the student identified above to register for this project under my supervision:


Unit chair/director/designate's name:

Unit chair/director/designate's signature - I certify that this project conforms to departmental requirements for 396 courses: