'Activation of orexinergic neurons in chick embryos' - NSCI 396 Undergraduate Research Project Application Form
INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted here. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature"). Ask your supervisor to sign her/his section near the bottom. Take it to the department* corresponding to the course number in Section A; this may or may not be your own department. (* EXCEPTIONS: For NSCI 396 and COGS 396, please bring it to the Interdisciplinary Programs Adviser in Dawson Hall.) Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and send a copy of this form (with signatures) to the Office for Undergraduate Research in Science (either fax, or internal mail to Dawson Hall 408-A, or PDF scan + email).
QUESTIONS OR FEEDBACK? Contact the Office for Undergraduate Research in Science.
Supervisor's Name: Maria Pompeiano
Supervisor's Email: maria [dot] pompeiano [at] mcgill [dot] ca
Supervisor's Phone: 514-398-6080
Supervisor's department: Psychology
Course number: NSCI 396 (Neuroscience)
Term: Winter 2012-2013
Project start date: Monday, January 7, 2013
Project end date: Tuesday, April 16, 2013
Project title: Activation of orexinergic neurons in chick embryos
Project description: Chick embryos show EEG signs of both stages of sleep: REM (rapid eye movement) sleep and NREM (non-REM) sleep in the last few days before hatching. A waking state is not seen in chick embryo EEG and seems to appear only at hatching. In the mature sleep-waking cycle, orexinergic neurons in the posterior hypothalamus regulate the appearance of the waking state by activating other arousal systems in the hypothalamus and brainstem (ex. noradrenergic locus coeruleus). Dr. Pompeiano's laboratory recently described the differentiation of orexinergic neurons at very early stages in the chick embryos. In the present project, we will try to correlate embryonic age with the activation of orexinergic neurons as seen by immediate-early gene expression. (ex. Egr1). We will test the hypothesis that orexinergic neurons or a subpopulation of orexinergic neurons will become activated as hatching approaches. This will be done by using fluorescent double-labeling immunohistochemical techniques in brain sections from chick embryos of different ages.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Grading scheme (The final report must be worth at least 50% of final grade): 50% lab performance; 50% final report
Project status: This project is taken. The professor has no more '396' projects this term.
How students can apply: N/A; this project is filled.
Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves handling chemicals.