'Expression, purification and preliminary structural characterization of MamE Protease' - ANAT 396 Undergraduate Research Project Application Form
INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted here. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature"). Ask your supervisor to sign her/his section near the bottom. Take it to the department* corresponding to the course number in Section A; this may or may not be your own department. (* EXCEPTIONS: For NSCI 396 and COGS 396, please bring it to the Interdisciplinary Programs Adviser in Dawson Hall.) Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and send a copy of this form (with signatures) to the Office for Undergraduate Research in Science (either fax, or internal mail to Dawson Hall 408-A, or PDF scan + email).
QUESTIONS OR FEEDBACK? Contact the Office for Undergraduate Research in Science.
Supervisor's Name: Justin Kollman
Supervisor's Email: justin [dot] kollman [at] mcgill [dot] ca
Supervisor's Phone: 514-398-4795
Supervisor's Website: kollmanlab.org
Supervisor's department: Anatomy and Cell Biology
Course number: ANAT 396 (Anatomy and Cell Biology)
Term: Winter 2012-2013
Project start date: Monday, January 7, 2013
Project end date: Tuesday, April 16, 2013
Project title: Expression, purification and preliminary structural characterization of MamE Protease
Project description: The project will involve optimizing expression of several constructs of a soluble form of the MamE protease, a critical component of bacterial magnetosomes. Following expression optimization, large scale cultures will be grown, and preliminary purification by nickel affinity chromatography and size exclusion chromatography will be carried out. Purified protein will be examined by negative stain EM to characterize the subunit organization and stoichiometry of MamE oligomers.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Grading scheme (The final report must be worth at least 50% of final grade): Final Report - 50%, lab performance 30%, final presentation 10%, participation in group meeting and journal club 10%.
Project status: This project is taken. The professor has no more '396' projects this term.
How students can apply: N/A; this project is filled.
Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Biohazardous substances; Handling chemicals.