'Deletion of the cpxA kinase sensor in Citrobacter rodentium' - MIMM 396 Undergraduate Research Project Application Form
INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature). Ask your supervisor to sign her/his section near the bottom. Take it to the department corresponding to the course number; this may or may not be your own department. Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science. The Office for Undergraduate Research in Science will later post the project online, indicating whether the project is open for students to apply or taken.
QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964
Supervisor's Name: Le Moual, Herve
Supervisor's Email: herve [dot] le-moual [at] mcgill [dot] ca
Supervisor's Phone: 514-398-6235
Supervisor's Website: http://www.mcgill.ca/microimm/members/professors/lemoual
Supervisor's department: Microbiology and Immunology
Course number: MIMM396 (Microbiology)
Term: Winter 2011-2012
Project start date: Monday, January 9, 2012
Project end date: Monday, April 16, 2012
Project title: Deletion of the cpxA kinase sensor in Citrobacter rodentium
Project description: Citrobacter rodentium is a bacterial pathogen that causes colonic hyperplasia in mice. Infection by C. rodentium is analogous to infections caused by the human enteric pathogens Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) that cause infantile diarrhoea and haemorrhagic colitis, respectively. Previous research showed that the CpxRA two-component regulatory system is important for bacterial virulence, since a cpxRA deletion C. rodentium strain was avirulent in mice. To generate a C. rodentium strain in which the CpxRA system is constitutively active, we intend to delete the cpxA gene only, whose protein product is known to inhibit the CpxR-mediated transcriptional regulation. The student will have to develop a good grasp of molecular biology under the supervision of a Ph.D. graduate student and generate this cpxA deletion mutant using the well-established sacB gene-based allelic exchange method.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages) graded by the supervisor and the course coordinator or the coordinator's delegate (50%).
Project status - This project is taken. The professor has no more '396' projects this term.
How students can apply: N/A; this project is filled.
Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Biohazardous substances; Handling chemicals
Student's McGill ID:
Student's Email (first [dot] last [at] mail [dot] mcgill [dot] ca):
Student's Level (U0 / U1 / U2 / U3):
Student's signature - I have not applied for another '396' course in this term:
Supervisor's signature - I give my permission for the student identified above to register for this project under my supervision:
Unit chair/director/designate's name: Gregory Marczynski
Unit chair/director/designate's signature - I certify that this project conforms to departmental requirements for 396 courses: