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'Interactions between the TBC-2 RabGAP and Rac GTPases' - ANAT 396 Undergraduate Research Project Application Form.

INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").

INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it from www.mcgill.ca/science/research/ours/396 after it has been posted. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature). Ask your supervisor to sign her/his section near the bottom. Take it to the department corresponding to the course number; this may or may not be your own department. Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.

INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science at 514-398-8102. The Office for Undergraduate Research in Science will later post the project online at www.mcgill.ca/science/research/ours/396/listing, indicating whether the project is open for students to apply or taken.

QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964

Supervisor's Name: Christian Rocheleau

Supervisor's Email: christian [dot] rocheleau [at] mcgill [dot] ca

Supervisor's Phone: (514) 398-3979

Supervisor's Website: www.rocheleaulab.com

Supervisor's department: Anatomy and Cell Biology

Course number: ANAT396 (Anatomy and Cell Biology)

Term: Fall 2011-2012

Project start date: Wednesday, September 1, 2011

Project end date: Tuesday, December 6, 2011

Project title: Interactions between the TBC-2 RabGAP and Rac GTPases

Project description: TBC-2 is a Rab5 GAP that localizes to late endosomes to regulate endosome maturation.  The mechanism regulating TBC-2 membrane localization is not known.  TBC-2 has been found to interact with the CED-10 Rac1 GTPase.  We hypothesize that CED-10 might recruit TBC-2 to late endosomes in a nucleotide dependent manner.  The student will determine if the physical interaction between CED-10 and TBC-2 is nucleotide dependent.  The student will express and purify GST tagged CED-10 proteins (wild-type and mutant) to perform pull-down assays, in the presence and absence of nucleotide analogs, using C. elegans lysates containing GFP::TBC-2.  The student will use western blot analysis with anti-GFP antibodies to test for interactions between GST-CED-10 and GFP::TBC-2 under different conditions.  In addition the student will use ced-10 RNAi and ced-10 mutants to test if ced-10 is required for membrane localization of GFP::TBC-2.  To do this the student will construct a ced-10(-); GFP::TBC-2 strain and analyze it and control strains by confocal microscopy.  These experiments will determine the in vivo importance of the CED-10-TBC-2 interactions and identify potential novel mechanisms by which Rac GTPases can regulate vesicular trafficking.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% on laboratory performance,  50% on a final written research report of at least 6 pages and a 30 minute  final oral presentation.

Project status - This project is: Taken. The professor has no more '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. Which of the following, if any, is involved?

  - Biohazardous substances

  - Handling chemicals

Student's Name:

Student's McGill ID:

Student's Email (first [dot] last [at] mail [dot] mcgill [dot] ca):

Student's Phone:

Student's Program:

Student's Level (U0 / U1 / U2 / U3):

Student's signature - I have not applied for another '396' course in this term:

Supervisor's signature - I give my permission for the student identified above to register for this project under my supervision:

Date:

Unit chair/director/designate's name:

Unit chair/director/designate's signature - I certify that this project conforms to departmental requirements for 396 courses:

Date: