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INSTRUCTIONS - PROFESSORS: Fill out Sections A & B then submit this form online. (You will receive an email copy of the form. The Office for Undergraduate Research in Science will also post the project online at http://www.mcgill.ca/science/research/ours/396/listing/, indicating whether the project is open for students to apply or taken.) |
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INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it from www.mcgill.ca/science/research/ours/396/listing/ after it has been posted. Either way, print this form. Complete and sign Section C on the hardcopy. Ask your supervisor to sign Section D. Take it to the department corresponding to the course number in Section A (this may or may not be your own department). Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective. |
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INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science at 514-398-8102. |
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QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964. |
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SECTION A: SUPERVISOR INFORMATION |
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Name: |
Gregory Marczynski |
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Email: |
gregory [dot] marczynski [at] mcgill [dot] ca |
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Phone: |
(514) 398-3917 |
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Website: |
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Supervisor's department: |
Microbiology and Immunology |
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Course number: |
MIMM 396 (Microbiology) |
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SECTION B: PROJECT INFORMATION |
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Term: |
Summer 2011 |
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Project start date: |
May 01, 2011 |
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Project end date: |
August 31, 2011 |
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Project title: |
Bacterial strains with defective chromosome replication protein binding sites. |
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Project description: |
The Caulobacter crescentus chromosome replication origin has one critical binding site for the DnaA replication protein. Conspicuous DNA mutations at this “DnaA box” abolish DnaA protein binding, these mutations prevent replication and they can not be placed by homologous recombination at their natural place on the chrosmome. This project has four phases: First, less server DnaA box changes will be made by a standard site-directed mutagenesis protocol. Second, these mutations will be tested for replication and recombination with the chromosome. The third phase will test if these mutations can be exchanged with the wild type binding sites. This standard procedure will therefore test if it is possible to create (as in the title) Caulobacter “strains with defective chromosome replication protein binding sites.” The fourth phase will test the cell cycle consequences and apply these strains to find genetic suppressors and presumably novel replication control proteins. This project will be considered satisfactory if phases one and two are completed and successful if phase three is also started and shows preliminary results. |
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Prerequisite: |
1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor. |
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Grading scheme (The final report must be worth at least 50% of final grade): |
Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages) graded by the supervisor and the course coordinator or the coordinator's delegate (50%). |
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Project status - This project is: |
Open to applicants |
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How students can apply: |
For MIMM: After all parts of this application form are completed, and the hard copy is signed by the professor and the student, bring this application form and your unofficial transcript to Prof. Gregory Marczynski during office hours, who will review/approve as the course coordinator for MIMM 396 (Microbiology) or MIMM 397 (Immunology). |
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Ethics, safety, and training |
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Which of the following, if any, is involved? |
None |
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Animal subjects |
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Human subjects |
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Biohazardous substances |
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Radioactive materials |
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Handling chemicals |
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Using lasers |
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Supervisors are responsible for the ethics and safety compliance of undergraduate students. |
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SECTION C: STUDENT INFORMATION |
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Do not complete this section unless/until the student is identified. |
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Name: |
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McGill ID: |
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Email (first [dot] last [at] mail [dot] mcgill [dot] ca): |
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Phone: |
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Program (E.g., B.Sc. Maj. Chem. Min. Biol.): |
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Level (U0 / U1 / U2 / U3): |
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Student signature - I have not applied for another '396' course in this term: |
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SECTION D: APPROVALS. |
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Do not complete this section unless/until the student is identified. |
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Supervisor: I give my permission for the student identified in section C to register for this project under my supervision. |
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Supervisor's signature: |
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Date: |
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Unit chair/director/designate: I certify that this project conforms to departmental requirements for 396 courses. |
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Unit chair/director/designate's name: |
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Unit chair/director/designate's signature: |
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Date: |
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