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Structure-Function Analysis of Rab35. Undergraduate Research Project Proposal Form.

INSTRUCTIONS - PROFESSORS: Fill out Sections A & B then submit this form online. (You will receive an email copy of the form. The Office for Undergraduate Research in Science will also post the project online, indicating whether the project is open for students to apply or taken.) DONE
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted. Either way, print this form. Complete and sign Section C on the hardcopy. Ask your supervisor to sign Section D. Take it to the department corresponding to the course number in Section A (this may or may not be your own department). Do not register for a '396' course on Minerva until you receive departmental permission.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science.
QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964.
Name: Peter S McPherson
Email: peter [dot] mcpherson [at] mcgill [dot] ca (peter dot mcpherson at mcgill dot ca)
Supervisor's department: Anatomy and Cell Biology
Course number: ANAT396 (Anatomy and Cell Biology)
Term: Fall 2010-2011
Project start date: Wednesday September 1 2010
Project end date: Friday December 3 2010
Project title: Structure-Function Analysis of Rab35
Project description: The DENN domain is an evolutionarily ancient protein module found in all eukaryotes. We have recently ascribed the first function to a DENN domain with the demonstration that the DENN domains of connecdenn 1, 2 and 3 (DENND1A, B and C) function as guanine nucleotide exchange factors (GEFs) towards Rab35, a GTPase involved in endosomal trafficking and actin dynamics (Allaire et al., 2010; Marat and McPherson, 2010). Rabs constitute the largest family of monomeric GTPases, with approximately 70 Rabs in human. Although Rabs share a great deal of homology, each Rab needs to be activated by a specific GEF to prevent aberrant activation. Rab35 is closely related to Rab1, yet the DENN domain does not have any GEF activity towards Rab1. We will generate chimeras between Rab35 and Rab1 to determine the critical residues within Rab35 that mediate the specificity of activation by the DENN domain.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Grading scheme (The final report must be worth at least 50% of final grade): 50% final written research report (minimum 10 pages), 50% laboratory performance as evaluated by the research supervisor
Project status - This project is: Taken. The professor has no more '396' projects this term.
How students can apply: N/A; this project is filled.
Ethics, safety, and training
Which of the following, if any, is involved? One or more of the following
Animal subjects [ ]
Human subjects [ ]
Biohazardous substances [ ]
Radioactive materials [ ]
Handling chemicals [x]
Using lasers [ ]
Supervisors are responsible for the ethics and safety compliance of undergraduate students.
Do not complete this section unless/until the student is identified.
McGill ID:  
Email (first [dot] last [at] mail [dot] mcgill [dot] ca):  
Program (E.g., B.Sc. Maj. Chem. Min. Biol.):  
Level (U0 / U1 / U2 / U3):  
Student signature - I have not applied for another '396' course in this term:  
Do not complete this section unless/until the student is identified.
Supervisor: I give my permission for the student identified in section C to register for this project under my supervision.
Supervisor's signature:  
Unit chair/director/designate: I certify that this project conforms to departmental requirements for 396 courses.
Unit chair/director/designate's name:  
Unit chair/director/designate's signature: