| INSTRUCTIONS - PROFESSORS: Fill out Sections A & B then submit this form online. (You will receive an email copy of the form. The Office for Undergraduate Research in Science will also post the project online, indicating whether the project is open for students to apply or taken.) DONE | |
| INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted. Either way, print this form. Complete and sign Section C on the hardcopy. Ask your supervisor to sign Section D. Take it to the department corresponding to the course number in Section A (this may or may not be your own department). Do not register for a '396' course on Minerva until you receive departmental permission. | |
| INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science at 514-398-8102. | |
| QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964. | |
| SECTION A: SUPERVISOR INFORMATION | |
| Name: | Sylvie Fournier |
| Email: | sylvie [dot] fournier [at] mcgill [dot] ca |
| Phone: | 514-398-7273 |
| Website: | |
| Supervisor's department: | Microbiology and Immunology |
| Supervisor's department (if none of the above) | |
| Course number: | MIMM397 (Immunology) |
| SECTION B: PROJECT INFORMATION | |
| Term: | Winter 2010 |
| Project start date: | January 4, 2010 |
| Project end date: | April 14, 2010 |
| Project title: | Characterization of CD8+ T cell hybridomas derived from the CNS |
| Project description: | We have generated B7.2 (CD86) transgenic mice that spontaneously develops a demyelinating disease in the CNS and PNS and in which CD8+ T cells play a pathogenic role. Using the CDR3 spectratyping analysis we have provided evidence for CD8+ T cell oligoclonal expansions within the CNS, suggesting that antigen (Ag)-specific CD8+ T cells are activated in situ by B7.2-expressing microglial cells. We study this animal model to characterize the mechanisms by which CD8+ T cells induce demyelination and axonal damage and to define the Ag specificity of the CD8+ T cells that are associated with the spontaneous development of a demyelinating disease. We have immortalized the CD8+ T cells that accumulate in the CNS of symptomatic animals through somatic cell hybridization with T cell lymphoma lines. The goal of this project is to grow these T cell hybridomas and to characterize them for TCR, CD4 and CD8 expression by 3-color flow cytometry. |
| Prerequisite: | 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor. |
| Other prerequisite, if applicable: | |
| Grading scheme (The final report must be worth at least 50% of final grade): | Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages) graded by the supervisor and the course coordinator or the coordinator's delegate (50%). |
| Other project information: | |
| Project status - This project is: | Taken. The professor has no more '396' projects this term. |
| How students can apply: | N/A; this project is filled. |
| If other, please specify: | |
| Ethics, safety, and training | |
| Which of the following, if any, is involved? | None |
| Animal subjects | [ ] |
| Human subjects | [ ] |
| Biohazardous substances | [ ] |
| Radioactive materials | [ ] |
| Handling chemicals | [ ] |
| Using lasers | [ ] |
| Supervisors are responsible for the ethics and safety compliance of undergraduate students. | |
| SECTION C: STUDENT INFORMATION | |
| Do not complete this section unless/until the student is identified. | |
| Name: | |
| McGill ID: | |
| Email (first [dot] last [at] mail [dot] mcgill [dot] ca): | |
| Phone: | |
| Program (E.g., B.Sc. Maj. Chem. Min. Biol.): | |
| Level (U0 / U1 / U2 / U3): | |
| Student signature - I have not applied for another '396' course in this term: | |
| SECTION D: APPROVALS. | |
| Do not complete this section unless/until the student is identified. | |
| Supervisor: I give my permission for the student identified in section C to register for this project under my supervision. | |
| Supervisor's signature: | |
| Date: | |
| Unit chair/director/designate: I certify that this project conforms to departmental requirements for 396 courses. | |
| Unit chair/director/designate's name: | |
| Unit chair/director/designate's signature: | |
| Date: | |