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Undergraduate Research Project Application Form

INSTRUCTIONS - PROFESSORS: Fill out Sections A & B then submit this form online. (You will receive an email copy of the form. The Office for Undergraduate Research in Science will also post the project online, indicating whether the project is open for students to apply or taken.) DONE
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted. Either way, print this form. Complete and sign Section C on the hardcopy. Ask your supervisor to sign Section D. Take it to the department corresponding to the course number in Section A (this may or may not be your own department). Do not register for a '396' course on Minerva until you receive departmental permission.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science.
QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964.
Name: David Hipfner
Email: david [dot] hipfner [at] ircm [dot] qc [dot] ca 
Phone: (514) 987-5508
Supervisor's department: Anatomy and Cell Biology
Supervisor's department (if none of the above)  
Course number: ANAT396 (Anatomy and Cell Biology)
Term: Winter 2010
Project start date: January 4, 2010
Project end date: April 14, 2010
Project title: A cell-culture-based screen for regulators of Moesin phosphorylation
Project description: Members of the Ezrin/Radixin/Moesin (ERM) family of proteins have emerged as important regulators of epithelial organization and cell morphology in vivo. ERM proteins act as regulated molecular crosslinkers that connect membrane proteins at the cell surface to the cortical F-actin cytoskeleton. By default, ERM proteins exist in a conformationally-inhibited state. Their activation is regulated by phosphorylation at a conserved C-terminal Thr residue. We have identified a protein kinase called Slik as the main regulator of ERM C-terminal phosphorylation in Drosophila. In order to identify other proteins that participate in ERM protein activation, we plan to do a genome-wide screen using a library of double-stranded RNAs targeting ~90% of Drosophila genes. The goal of this project will be to develop and validate a cell-culture-based screening assay that will allow changes in Moesin phosphorylation to be measured, based on double-immunofluorescence labeling of phosphorylated Moesin and a reference protein. The project will involve cell culture, immunofluorescence in 96-well plates, fluorescence microscopy, and image analysis using Metamorph software.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Other prerequisite, if applicable:  
Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages) graded by the supervisor and the course coordinator or the coordinator's delegate (50%).
Other project information:
Project status - This project is: Taken; however students may contact the professor to discuss other possible '396' projects this term.
How students can apply: N/A; this project is filled.
If other, please specify:  
Ethics, safety, and training
Which of the following, if any, is involved? One or more of the following
Animal subjects [ ]
Human subjects [ ]
Biohazardous substances [x]
Radioactive materials [ ]
Handling chemicals [x]
Using lasers [ ]
Supervisors are responsible for the ethics and safety compliance of undergraduate students.
Do not complete this section unless/until the student is identified.
McGill ID:
Email (first [dot] last [at] mail [dot] mcgill [dot] ca):
Program (E.g., B.Sc. Maj. Chem. Min. Biol.):  
Level (U0 / U1 / U2 / U3):  
Student signature - I have not applied for another '396' course in this term:  
Do not complete this section unless/until the student is identified.
Supervisor: I give my permission for the student identified in section C to register for this project under my supervision.
Supervisor's signature:  
Unit chair/director/designate: I certify that this project conforms to departmental requirements for 396 courses.
Unit chair/director/designate's name:  
Unit chair/director/designate's signature: