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Real Time PCR/Quantitative PCR

There are two Real Time PCR Thermal Cyclers available: the Cepheid Smart Cycler II from Fisher Scientific and the Rotor Gene from MBI.

The Cepheid Smart Cycler II has 16 independent wells to accommodate 16 different PCR protocols. It can be use to optimize the PCR reaction by running a temperature gradient for example.

The Rotor Gene unit holds 36 x 0.2ml tubes or 72 x 0.1ml tubes. Once the conditions are optimized, a large number of samples can be run at the same time (endogenous control, standard curve, unknown samples, NTC).

The fees for both instruments are 35$/run, tubes not provided or 40$/run, tubes provided.