Confocal Imaging Facility
Located in room D28 of the Lyman Duff Medical Building, the Department of Microbiology and Immunology is equipped with a Fluoview 1000 confocal microscope from Olympus. The FV1000 contains 458/488/515/543/633nm lasers allowing for simultaneous multi-colour imaging and 10-100x objectives to meet a range of experimental needs.
Confocal microscopy provides several advantages over conventional epifluorescence microscopy including increased image resolution and three-dimensional rendering. For a tutorial on the basics of immunofluorescence microscopy, go to: http://www.physics.emory.edu/~weeks/confocal/