Regulation of gene expression in eukaryotes
Accepting graduate students
Hormones & Cancer Research Unit
Department of Medicine
Royal Victoria Hospital
687 Pine Avenue West
Montreal, QC H3A 1A1
Tel: (514) 934-1934 x.35842
Fax: (514) 843-2819
bernard [dot] turcotte [at] mcgill [dot] ca (Email)
Research in my laboratory relates to functional genomics in budding yeast. We are focusing on the Gal4 family of transcriptional regulators which includes over 50 members. The function of many of these Gal4 members is unknown or poorly defined. To better understand the role of these transcriptional regulators and identify their target genes, we are using various approaches including genetics, molecular biology and whole-genome analysis of gene expression with DNA microarrays. We are also determining the role of related Gal4 members in conferring resistance to antifungal drugs in the human pathogen Candida albicans. Another project is aimed at studying the mechanism of action of the anti-cancer drug tirapazamine.
Selected Recent Publications
Soontorngun, N., M. Larochelle, S. Drouin, F. Robert and B. Turcotte (2007) "Regulation of gluconeogenesis in Saccharomyces cerevisiae is mediated by activator and repressor functions of Rds2." Mol. Cell. Biol. 27: 7895-7905.
MacPherson, S., M. Larochelle and B. Turcotte (2006) "A fungal family of transcriptional regulators: the zinc cluster proteins." Microbiol. Mol. Biol. Rev. 17: 583-604.
Larochelle, M., S. Drouin, F. Robert and B. Turcotte (2006) "Oxidative stress-activated zinc cluster protein Stb5 has dual activator/repressor functions required for pentose phosphate pathway regulation and NADPH production." Mol. Cell. Biol. 26: 6690-6701.
Hellauer, K., G. Lesage, A.-M. Sdicu and B. Turcotte (2005) "Large-scale analysis of genes that alter sensitivity to the anti-cancer drug tirapazamine in Saccharomyces cerevisiae." Mol. Pharm. 68: 1365-1375.
MacPherson, S., B. Akache, S. Weber, X. De Deken, M. Raymond and B. Turcotte (2005) "The Candida albicans zinc cluster protein Upc2p confers resistance to antifungal drugs and is an activator of ergosterol biosynthetic genes." Antimicrob. Agents Chemother. 49: 1745-52.
Akache, B., S. MacPherson, M.-A. Sylvain and B. Turcotte (2004) "Complex interplay among regulators of drug resistance in S. cerevisiae." J. Biol. Chem. 279: 27855-27860.
Akache B, Turcotte B (2002) "New regulators of drug sensitivity in the family of yeast zinc cluster proteins." J Biol Chem. 277(24):21254-60.
Hellauer K, Akache B, MacPherson S, Sirard E, Turcotte B (2002) "Zinc cluster protein Rdr1p is a transcriptional repressor of the PDR5 gene encoding a multidrug transporter." J Biol Chem. 277(20):17671-6.
Akache B, Wu K, Turcotte B (2001) "Phenotypic analysis of genes encoding yeast zinc cluster proteins." Nucleic Acids Res. 29(10):2181-90.
Hellauer K, Sirard E, Turcotte B (2001) "Decreased expression of specific genes in yeast cells lacking histone H1." J Biol Chem. 276(17):13587-92.