Assistant Professor
Gene transfer, fluorescent labeling, nanoparticles
Accepting graduate students (see post-doc opening )
Biomedical Engineering
Lyman Duff Medical Building
Room 310
3775 University St.
Montreal, QC H3A 2B4
Tel: (514) 398-8372
Fax: (514) 398-7461
jay [dot] nadeau [at] mcgill [dot] ca (Email)
Website: audilab.bmed.mcgill.ca/~nadeau/
Research Orientations
Cell labeling with nanoparticles, mechanisms of nanoparticle import, toxicity of quantum dots and Fe crystals to cells.
I am interested in the effects of alterations of electrical excitability on the formation and behavior of neuronal synapses. To this end, I am developing a variety of tools for genetic modification and fluorescent labeling of neurons (and other cells). Specific interests include manufacture of quantum dots for use as potential voltage-sensitive probes, introduction of bacterial sodium channels into neurons using viral vectors, and genetic suppression of sodium channel function using the protein NRSF (neuron-restrictive silencer factor). I am also interested in the single-channel properties of sodium channels and in developing methods to study them for long periods (days to weeks).
Selected Recent Publications
Kleopfer JA, Mielke R, Nadeau JL. "Uptake of CdSe and CdSe/Zns quantum dots into bacteria via purine-dependent mechanisms." Applied and Environmental Microbiology. Submitted.
Kloepfer JA, Cohen N, Nadeau JL. "FRET between quantum dots and lipid- and water-soluble dyes." Journal of Physical Chemistry B. In press.
Kloepfer JA, Mielke RE, Wong MS, Nealson KH, Stucky G, Nadeau JL. "Quantum dots as strain- and metabolism-specific microbiological labels." Appl Environ Microbiol 2003 Jul;69(7):4205-13.
Nadeau JL. "Lentivirus Gene Engineering Protocols: Hippocampal Neurons." Methods Mol Biol. 2003;229:141-54. No abstract available.