Nobel Prize for GFP
Osamu Shimomura, of the Marine Biological Laboratory, Woods Hole, Martin Chalfie of Columbia University, New York, and Roger Tsien of the University of California, San Diego, share the prize. Read More
Generous Gene Discoverer Dr. Prasher Left out.
My proteins are "co-localized" in focal adhesions: OR ARE THEY ?
Many new ultra-high resolution light microscopy techniques have been developed in the past few years (James H. Rice Review). One of these recently developed techniques called PALM (Photoacitvated Localization Microscopy) was used for dual colour imaging in focal adhesions. The images below show an overlay of the same region of the same cell (the adaptor protein paxillin localization is shown in green and the actin binding protein zyxin localization is shown in red) imaged using standard TIRF fluorescence (upper two images) followed by the new PALM technique (lower images).
Keep this in mind when you are doing those co-localization experiments!