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Nobel Prize for GFP

Osamu Shimomura, of the Marine Biological Laboratory, Woods Hole, Martin Chalfie of Columbia University, New York, and Roger Tsien of the University of California, San Diego, share the prize. Read More

Generous Gene Discoverer Dr. Prasher Left out.

My proteins are "co-localized" in focal adhesions:
OR ARE THEY ?

Many new ultra-high resolution light microscopy techniques have been developed in the past few years (James H. Rice Review). One of these recently developed techniques called PALM (Photoacitvated Localization Microscopy) was used for dual colour imaging in focal adhesions. The images below show an overlay of the same region of the same cell (the adaptor protein paxillin localization is shown in green and the actin binding protein zyxin localization is shown in red) imaged using standard TIRF fluorescence (upper two images) followed by the new PALM technique (lower images).

Keep this in mind when you are doing those co-localization experiments!

The McGill LSC Imaging Facility was made possible through a grant from the Canadian Foundation for Innovation (CFI) and was established in 2003. The mandate of the LSC Imaging Facility is to provide a state of the art light microscopy imaging facility with advanced technical expertise in order to enhance the level of research for the community at McGill and in the scientific community outside of McGill.

Claire Brown's Microscopy Pitfalls Poster:

The major goals of the facility include:

• Streamline access to advanced light microscopy imaging equipment available at McGill University.
• Direct researchers to the appropriate equipment and techniques to address their specific research questions.
• To acquire new equipment in order to keep up with advances in imaging to provide state of the art technologies and instrumentation to researchers.
• Develop our own advances in image acquisition and analysis techniques giving researchers unique tools to answer complex biological questions.
• Develop a network of image analysis workstations with the latest high end image analysis software packages.
• Provide researchers with training in advance imaging techniques and software analysis tools.
• Partner with companies to develop both short and indepth training courses for efficient technology transfer to researchers.

What we do:

• Confocal Microscopy
• Multiple fluorophore imaging
• TIRF Imaging
• 3D Imaging
• 4D Dynamic Live Cell Imaging
• Laser micro-dissection (PALM)
• Protein diffusion/interactions in living cells (FCS)
• Cell surface imaging with <100 nM z resolution (TIRF)
• FRET
• FRAP
• De-convolution
• FCS
• Image Correlation Microscopy (ICS, RICS, STICS)