Core Equipment and Fees

Equipment

Function

Fees

BD FACS Calibur

(4 Color flow cytometer)

Training

$100/hr (Min. 3hrs)

User Fee

$40/hr

Analysis Assistance

$40/hr

Assay Development

$40/hr

Cyan ADP Analyzer

(9 color flow cytometer)

Training

$100/hr (Min. 3hrs)

User Fee

$40/hr

Analysis Assistance

$40/hr

Assay Development

$40/hr

Luminex Multiplex Plate Reader

User Fee

$40/hr

ImageXpress - MetaXpress

User Fee

$40/hr

Victor 3 Plate Reader

User Fee

$40/hr

 

To fulfill its main mission as a translational program at the MNI, the ETP has been establishing several platforms that will also serve as core facilities to the MNI and external communities.

 

Core Facility Equipment

Several core instruments are available through the ETP, including:

FACSCalibur: State of the art flow cytometry instrument and software. Proof-of-principle flow cytometry studies have validated the ability to measure cytokine regulation in small, biologically distinct human cell subsets within a mixed population of cells. This is proving of particular utility for limiting sample volumes, for example, those obtained from the Canadian pediatric Demyelinating Disease study described below. Flow cytometry training is regularly provided by the ETP to personnel of MNI laboratories.
Click here to reserve time on the FACSCalibur.

Beckman Coulter Cyan Flow Cytometer (please contact the ETP for special training):

  • 11 standard parameters and 9 colors
  • multiple laser excitation sources to analyze biological cells, beads or microscopic particles
  • pulse processing speed up to 70,000 events/s
  • high acquisition and analysis capability of up to 100,000,000 event data files.

Click here to reserve time on the Beckman Coulter Cyan Flow Cytometer.

Luminex: A microsphere based multiplexing system that allows for intensive protein and gene expression profiling at a fraction of the labor and materials cost. This system has already been used to develop a profile of potential biomarkers in PD-ADS and other patient cohorts studies by multiple MNI and external investigators.
Click here to reserve time on the Luminex.

 

Victor 3: The VICTOR™ 3 is an instrument designed for running cost-effective cellular assays and screening technologies. The multilabel, multi-task plate readers can run multiple assay technologies such as time-resolved fluorescence assays on a single platform. The ETP Victor 3 has an added injector capability of rapid delivery of compounds to particular wells, and also has a heated stage to maintain plates at specific temperatures.
Click here to reserve time on the Victor3.

 

ImageXpress: A rapid, automated cellular image acquisition system. The ImagXpress allows for morphological analysis of the complex cellular structure of neurons and oligodendrocytes. The high throughput capabilities of this integrated hardware and software technology has had an impact on both industry-related and MNI investigator-initiated studies.
Click here to reserve time on the ImageXpress.