Pavillon de Sciences
1200 rue St. Alexandre
Montreal, Quebec H3B 3H5
Tel.: 514-9873000, extension 1918.
scorza [dot] tatiana [at] uqam [dot] ca (Email)
Two research projects are being developed in my laboratory:
- A first project concerns the study of immunosuppressive mechanisms induced by Plasmodium spp., affecting the function of antigen presenting cells (APCs) and the activation of T cells. Parasites (P. falciparum, P. chabaudi adami) are isolated from infected red blood cells using standards methods. Plasmodium proteins are extracted and assessed for their immunosuppressive activity on T cell hybridomas and T cells. In addition we are interested in evaluating the effect of these suppressive metabolites on the priming of CD8+T cell responses (cytotoxicity and IFN-alpha secretion) by DNA vaccines in vivo and in vitro. We also focus on the identification of immunomodulatory factors secreted by APCs that contribute to the downregulation of T cell responses. In this context, conditioned media from the culture of APCs with Plasmodium-derived suppressive proteins and /or metabolites are analyzed by mass spectrometry. Special attention is given to the malaria pigment (hemozoin), which has been shown to increase the peroxydation of lipids in human and murine macrophages, and which can affect the activation of T cells.
- A second research project concerns the development of new therapeutic strategies to treat cutaneous and visceral forms of Leishmaniasis using murine laboratory models. This project involves the collaboration with McGill University and with the Centre for Parasitological Research José W. Torrealba, Trujillo-Venezuela.
Malu DT, Bélanger B, Desautels F, Kelendji K, Dalko E, Sanchez-Dardon J, Leng L, Bucala R, Satoskar AR, Scorza T.
Macrophage migration inhibitory factor: a downregulator of early T cell-dependent IFN-gamma responses in Plasmodium chabaudi adami (556 KA)-infected mice. J Immunol. 2011 Jun 1;186(11):6271-9. Epub 2011 Apr 25.
Cambos M, Scorza T.
Robust erythrophagocytosis leads to macrophage apoptosis via a hemin-mediated redox imbalance: role in hemolytic disorders. J Leukoc Biol. 2011 Jan;89(1):159-71. Epub 2010 Sep 30.
Cambos M, Bazinet S, Abed E, Sanchez-Dardon J, Bernard C, Moreau R, Olivier M, Scorza T.
The IL-12p70/IL-10 interplay is differentially regulated by free heme and hemozoin in murine bone-marrow-derived macrophages. Int J Parasitol. 2010 Aug 1;40(9):1003-12. Epub 2010 Mar 6.
Scorza T, Grubb K, Cambos M, Santamaria C, Tshikudi Malu D, Spithill TW.
Vaccination with a Plasmodium chabaudi adami multivalent DNA vaccine cross-protects A/J mice against challenge with P. c. adami DK and virulent Plasmodium chabaudi chabaudi AS parasites. Int J Parasitol. 2008 Jun;38(7):819-27. Epub 2007 Nov 20.
Cambos M, Bélanger B, Jacques A, Roulet A, Scorza T.
Natural regulatory (CD4+CD25+FOXP+) T cells control the production of pro-inflammatory cytokines during Plasmodium chabaudi adami infection and do not contribute to immune evasion. Int J Parasitol. 2008 Feb;38(2):229-38. Epub 2007 Jul 26.
Scorza T, Grubb K, Smooker P, Rainczuk A, Proll D, Spithill TW.
Induction of strain-transcending immunity against Plasmodium chabaudi adami malaria with a multiepitope DNA vaccine. Infect Immun. 2005 May;73(5):2974-85.
Rainczuk A, Scorza T, Spithill TW, Smooker PM.
A bicistronic DNA vaccine containing apical membrane antigen 1 and merozoite surface protein 4/5 can prime humoral and cellular immune responses and partially protect mice against virulent Plasmodium chabaudi adami DS malaria. Infect Immun. 2004 Oct;72(10):5565-73.