Institute of Parasitology
James McGill Professor,
Macdonald Campus, McGill University
21,111 Lakeshore Road
Ste. Anne de Bellevue, Qc, H9X 3V9
Anthelmintic resistance: Our group is determining the molecular pharmacology and genetics of resistance to antiparasitic drugs. Resistance has become a major problem for control programs in a number of parasitic diseases. We are focusing on possible resistance development to macrocyclic lactone, benzimidazole and other drugs which are used for the control of human filarial diseases such as onchocerciasis (ivermectin), and lymphatic filariasis (albendazole, ivermectin and diethylcarbamazole), and on resistance to avermectins (e.g. ivermectin, abamectin, doramectin, etc.) and milbemycins (moxidectin) in veterinary nematode parasites such as Haemonchus contortus and Cooperia oncophora. In these different drug/parasite systems a number of genes have been identified as being associated with selection for anthelmintic resistance. Specific mutations have been identified in these genes and the effects of these mutations on the functioning of the gene products are being determined. This information is allowing us to develop DNA based probes for resistance associated mutations which can be used to determine the frequency of resistance mutations in parasite populations and to participate in epidemiological studies in the field. We collaborate with a number of laboratories in developing countries and international organizations for the studies on parasitic organisms of humans and with international laboratories and companies for the veterinary parasitic investigations. The research involves laboratory molecular biology studies and field work on control and epidemiology.
Barrère V, Alvarez L, Suarez G, Ceballos L, Moreno L, Lanusse C, Prichard RK.Relationship between increased albendazole systemic exposure and changes in single nucleotide polymorphisms on the β-tubulin isotype 1 encoding gene in Haemonchus contortus. Vet Parasitol. 2011 Nov 28. [Epub ahead of print]
Geary TG, Bourguinat C, Prichard RK.
Evidence for macrocyclic lactone anthelmintic resistance in Dirofilaria immitis. Top Companion Anim Med. 2011 Nov;26(4):186-92. Review.
Osei-Atweneboana MY, Awadzi K, Attah SK, Boakye DA, Gyapong JO, Prichard RK.
Phenotypic evidence of emerging ivermectin resistance in Onchocerca volvulus. PLoS Negl Trop Dis. 2011 Mar 29;5(3):e998.
Bourguinat C, Keller K, Prichard RK, Geary TG.
Genetic polymorphism in Dirofilaria immitis. Vet Parasitol. 2011 Mar 22;176(4):368-73. Epub 2011 Jan 19.
Bourguinat C, Keller K, Blagburn B, Schenker R, Geary TG, Prichard RK.
Correlation between loss of efficacy of macrocyclic lactone heartworm anthelmintics and P-glycoprotein genotype. Vet Parasitol. 2011 Mar 22;176(4):374-81. Epub 2011 Jan 19.
Rao VT, Forrester SG, Keller K, Prichard RK.
Localisation of serotonin and dopamine in Haemonchus contortus. Int J Parasitol. 2011 Feb;41(2):249-54. Epub 2010 Sep 29.
Beech RN, Skuce P, Bartley DJ, Martin RJ, Prichard RK, Gilleard JS.
Anthelmintic resistance: markers for resistance, or susceptibility? Parasitology. 2011 Feb;138(2):160-74. Epub 2010 Sep 9. Review.
Bourguinat C, Kamgno J, Boussinesq M, Mackenzie CD, Prichard RK, Geary TG.
Analysis of the mdr-1 gene in patients co-infected with Onchocerca volvulus and Loa loa who experienced a post-ivermectin serious adverse event. Am J Trop Med Hyg. 2010 Jul;83(1):28-32.