2705, boulevard Laurier, RC-709
CANADA G1V 4G2
Tel:+1 418-525-4444, poste 48184
marc [dot] ouellette [at] crchul [dot] ulaval [dot] ca (Email)
My program of reseach has been devoted to the study of antimicrobial resistance mechanisms in the parasite Leishmania and more recently in the bacterium Streptococcus pneumoniae. The parasite Leishmania is a priority of the World Health Organization and one of the six top gun pathogens in tropical Medicine. Pentavalent antimony is the first line treatment against leishmaniasis but resistance has now reached an epidemic level in some part of the world. The work of Dr Ouellette on antimony resistance has highlighted the role of membrane transporters, including ABC-type proteins, and the thiol metabolism (cysteine, glutathione, trypanothion) as key players in resistance. Leishmania parasites are auxotrophs for folic acid. Studying this metabolic pathway and linked pathways (pterine and methionine) has pinpointed novel membrane transporters and enzymes that could serve as new targets. More recently, Dr Ouellette’s research interest has also included antibiotic resistance in the bacterium S. pneumoniae. Combining transcriptomics (microarrays) and proteomics techniques, his team is now studying resistance mechanisms in pathogens in a global scale.
Raven JF, Ouellette M.
The Canadian Institutes of Health Research institute of infection and immunity response to the threat of antimicrobial resistance. Rev Panam Salud Publica. 2011 Dec;30(6):663-4.
Coelho AC, Boisvert S, Mukherjee A, Leprohon P, Corbeil J, Ouellette M.
Multiple Mutations in Heterogeneous Miltefosine-Resistant Leishmania major Population as Determined by Whole Genome Sequencing. PLoS Negl Trop Dis. 2012 Feb;6(2):e1512. Epub 2012 Feb 14.
Plourde M, Coelho A, Keynan Y, Larios OE, Ndao M, Ruest A, Roy G, Rubinstein E, Ouellette M.
Genetic polymorphisms and drug susceptibility in four isolates of Leishmania tropica obtained from Canadian soldiers returning from Afghanistan. PLoS Negl Trop Dis. 2012 Jan;6(1):e1463. Epub 2012 Jan 17.
Fani F, Leprohon P, Légaré D, Ouellette M.
Whole genome sequencing of penicillin-resistant Streptococcus pneumoniae reveals mutations in penicillin-binding proteins and in a putative iron permease. Genome Biol. 2011 Nov 22;12(11):R115. [Epub ahead of print].
Billal DS, Feng J, Leprohon P, Legare D, Ouellette M.
Whole genome analysis of linezolid resistance in Streptococcus pneumoniae reveals resistance and compensatory mutations. BMC Genomics. 2011 Oct 17;12(1):512. [Epub ahead of print]
Raymond F, Boisvert S, Roy G, Ritt JF, Légaré D, Isnard A, Stanke M, Olivier M, Tremblay MJ, Papadopoulou B, Ouellette M, Corbeil J.
Genome sequencing of the lizard parasite Leishmania tarentolae reveals loss of genes associated to the intracellular stage of human pathogenic species. Nucleic Acids Res. 2012 Feb;40(3):1131-47. Epub 2011 Oct 13.
Leprohon P, Légaré D, Ouellette M.
ABC transporters involved in drug resistance in human parasites. Essays Biochem. 2011 Sep 7;50(1):121-44. Review.
Feng J, Billal DS, Lupien A, Racine G, Winstall E, Légaré D, Leprohon P, Ouellette M.
Proteomic and transcriptomic analysis of linezolid resistance in Streptococcus pneumoniae.J Proteome Res. 2011 Oct 7;10(10):4439-52. Epub 2011 Sep 13.