21,111 Lakeshore Road
Ste. Anne de Bellevue, Qc, H9X 3V9
armando [dot] jardim [at] mcgill [dot] ca (Email)
Leishmania and related trypanosomatids have a specialized organelle called a glycosome that compartmentalizes enzymes essential for a variety of enzymes essential for metabolic processes such as glycolysis, fatty acid b-oxidation, purine salvage, pyrimidine biosynthesis, lipid biosynthesis, and carbon dioxide fixation. These glycosomal proteins are synthesized in the cytosolic and imported across the glycosome membrane by a yet unknown mechanism. My lab uses a multidisciplinary approach that exploits protein chemistry, bioinformatics, molecular biology, immunocytochemistry, and genetic techniques to investigated the molecular processes involved in the targeting and import of proteins into the glycosome. An ultimate goal of this work is to identify potential protein targets that may be used to develop new antiparasitic chemotherapeutic agents.
Boitz JM, Strasser R, Hartman CU, Jardim A, Ullman B.
Adenine Aminohydrolase from Leishmania donovani: Unique enzyme in parasite purine metabolism. J Biol Chem. 2012 Mar 2;287(10):7626-39. Epub 2012 Jan 11.
Fulwiler AL, Boitz JM, Gilroy C, Yates PA, Jardim A, Ullman B.
IMP dehydrogenase deficiency in Leishmania donovani causes a restrictive growth phenotype in promastigotes but is not essential for infection in mice. Mol Biochem Parasitol. 2011 Dec;180(2):123-6. Epub 2011 Sep 3.
Hassani K, Antoniak E, Jardim A, Olivier M.
Temperature-induced protein secretion by Leishmania mexicana modulates macrophage signalling and function. PLoS One. 2011 May 3;6(5):e18724.
Feng X, Feistel T, Buffalo C, McCormack A, Kruvand E, Rodriguez-Contreras D, Akopyants NS, Umasankar PK, David L, Jardim A, Beverley SM, Landfear SM.
Remodeling of protein and mRNA expression in Leishmania mexicana induced by deletion of glucose transporter genes. Mol Biochem Parasitol. 2011 Jan;175(1):39-48. Epub 2010 Sep 24.
Chavda S, Babu B, Yanow SK, Jardim A, Spithill TW, Kiakos K, Kluza J, Hartley JA, Lee M.
A novel achiral seco-cyclopropylpyrido[e]indolone (CPyI) analog of CC-1065 and the duocarmycins: synthesis, DNA interactions, in vivo anticancer and anti-parasitic evaluation.Bioorg Med Chem. 2010 Jul 15;18(14):5016-24. Epub 2010 Jun 4.
Ullman B, Cyr N, Choi K, Jardim A.
Acidic residues in the purine binding site govern the 6-oxopurine specificity of the Leishmania donovani xanthine phosphoribosyltransferase. Int J Biochem Cell Biol. 2010 Feb;42(2):253-62. Epub 2009 Oct 25.