Canadian Research Chair in Structural Biology
Department of Biochemistry
Francesco Bellini Life Sciences Building
3649 Promenade Sir William Osler
Office: Room 466; Lab: Room 468/470
Montreal, QC H3G 0B1
Tel: 514-398-8795; Lab: 514-398-8781/ 2177
albert [dot] berghuis [at] mcgill [dot] ca (Email)
X-ray crystallographic studies of proteins, particularly enzymes that can be exploited as targets for drug development. Research efforts have concentrated on structure determination of enzymes that can be exploited as targets for antibiotics and antimycotics, and bacterial enzymes responsible for antibiotic resistance. Furthermore, efforts are ongoing to exploit the three-dimensional structural information obtained for the structure-based design of novel antimicrobial agents, using computational methods.
Lin YS, Park J, Deschutter JW, Huang XF, Berghuis AM, Sebag M, Tsantrizos YS.
Design and Synthesis of Active Site Inhibitors of the Human Farnesyl Pyrophosphate Synthase - Apoptosis and Inhibition of ERK Phosphorylation in Multiple Myeloma Cells. J Med Chem. 2012 Mar 5. [Epub ahead of print]
Shi K, Berghuis AM.
Structural basis for the dual nucleotide selectivity of aminoglycoside 2"-phosphotransferase IVa provides insight on determinants of nucleotide specificity of aminoglycoside kinases. J Biol Chem. 2012 Feb 24. [Epub ahead of print]
Caldwell SJ, Berghuis AM.
Small-angle X-ray scattering analysis of the bifunctional antibiotic resistance enzyme aminoglycoside (6' ) acetyltransferase-Ie/aminoglycoside (2'') phosphotransferase-Ia reveals a rigid solution structure. Antimicrob Agents Chemother. 2012 Jan 30. [Epub ahead of print]
Yachnin BJ, Colin DY, Volpato JP, Ebert M, Pelletier JN, Berghuis AM.
Novel crystallization conditions for tandem variant R67 DHFR yield a wild-type crystal structure. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Nov 1;67(Pt 11):1316-22. Epub 2011 Oct 25.
Shi K, Houston DR, Berghuis AM.
Crystal structures of antibiotic-bound complexes of aminoglycoside 2''-phosphotransferase IVa highlight the diversity in substrate binding modes among aminoglycoside kinases. Biochemistry. 2011 Jul 19;50(28):6237-44. Epub 2011 Jun 27.
Fong DH, Xiong B, Hwang J, Berghuis AM.
Crystal structures of two aminoglycoside kinases bound with a eukaryotic protein kinase inhibitor. PLoS One. 2011 May 9;6(5):e19589.
Freiburger LA, Baettig OM, Sprules T, Berghuis AM, Auclair K, Mittermaier AK.
Competing allosteric mechanisms modulate substrate binding in a dimeric enzyme. Nat Struct Mol Biol. 2011 Mar;18(3):288-94. Epub 2011 Jan 30.
Zhang Z, Mui MZ, Chan F, Roopchand DE, Marcellus RC, Blanchette P, Li S, Berghuis AM, Branton PE.
Genetic analysis of B55alpha/Cdc55 protein phosphatase 2A subunits: association with the adenovirus E4orf4 protein. J Virol. 2011 Jan;85(1):286-95. Epub 2010 Nov 3.