Associate Professor
Canadian Research Chair in Structural Biology
Department of Biochemistry
McGill University and Genome
Quebec Innovation Centre
740 Dr. Penfield, Room 5202
Montreal, QC H3A 1A4
Tel: (514) 398-8795
Fax: (514) 398-2036
albert [dot] berhuis [at] mcgill [dot] ca (Email)
Research Interests
X-ray crystallographic studies of proteins, particularly enzymes that can be exploited as targets for drug development. Research efforts have concentrated on structure determination of enzymes that can be exploited as targets for antibiotics and antimycotics, and bacterial enzymes responsible for antibiotic resistance. Furthermore, efforts are ongoing to exploit the three-dimensional structural information obtained for the structure-based design of novel antimicrobial agents, using computational methods.
Selected Publications
- Volpato JP, Yachnin BJ, Blanchet J, Guerrero V, Poulin L, Fossati E, Berghuis AM, Pelletier JN. (2009)
- Multiple conformers in active site of human dihydrofolate reductase F31R/Q35E double mutant suggest structural basis for methotrexate resistance. J Biol Chem. 284(30):20079-89.
- Fong DH, Berghuis AM. (2009)
- Structural basis of APH(3')-IIIa-mediated resistance to N1-substituted aminoglycoside antibiotics. Antimicrob Agents Chemother. 53(7):3049-55.
- Miller WL, Matewish MJ, McNally DJ, Ishiyama N, Anderson EM, Brewer D, Brisson JR, Berghuis AM, Lam JS. (2008)
- Flagellin glycosylation in Pseudomonas aeruginosa PAK requires the O-antigen biosynthesis enzyme WbpO. J Biol Chem. 283(6):3507-18.
- Korczynska M, Mukhtar TA, Wright GD, Berghuis AM.(2007)
- Structural basis for streptogramin B resistance in Staphylococcus aureus by virginiamycin B lyase. Proc Natl Acad Sci U S A. 104: 10388-93.
- Nascimento M, Zhang WW, Ghosh A, Houston DR, Berghuis AM, Olivier M, Matlashewski G. (2006)
- Identification and characterization of a protein-tyrosine phosphatase in Leishmania: Involvement in virulence. J Biol Chem. 281: 36257-68.
- Ishiyama N, Creuzenet C, Miller WL, Demendi M, Anderson EM, Harauz G, Lam JS, Berghuis AM. (2006)
- Structural studies of Flaa1 from Helicobacter pylori reveal the mechanism for inverting 4,6-dehydratase activity. J Biol Chem.
- Fong DH, Yim VC, D'Elia MA, Brown ED, Berghuis AM. (2006)
- Crystal structure of CTP:glycerol-3-phosphate cytidylyltransferase from Staphylococcus aureus: examination of structural basis for kinetic mechanism. Biochim Biophys Acta. 1764: 63-9.
- Lemke CT, Hwang J, Xiong B, Cianciotto NP, Berghuis AM. (2005)
- Crystallization and preliminary crystallographic analysis of an aminoglycoside kinase from Legionella pneumophila. Acta Crystallograph Sect F Struct Biol Cryst Commun. 61: 606-8.
- Mirza IA, Nazi I, Korczynska M, Wright GD, Berghuis AM. (2005)
- Crystal structure of homoserine transacetylase from Haemophilus influenzae reveals a new family of alpha/beta-hydrolases. Biochemistry. 44: 15768-73.
- Gao F, Yan X, Baettig OM, Berghuis AM, Auclair K. (2005)
- Regio- and chemoselective 6'-N-derivatization of aminoglycosides: bisubstrate inhibitors as probes to study aminoglycoside 6'-N-acetyltransferases. Angew Chem Int Ed Engl. 44: 6859-62.
- Burk DL, Xiong B, Breitbach C, Berghuis AM. (2005)
- Structures of aminoglycoside acetyltransferase AAC(6')-Ii in a novel crystal form: structural and normal-mode analyses. Acta Crystallogr D Biol Crystallogr. 61:1 273-9.
- Christensen KE, Mirza IA, Berghuis AM, Mackenzie RE. (2005)
- Magnesium and phosphate ions enable NAD binding to methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase. J Biol Chem. 280: 34316-23.
- Demendi M, Ishiyama N, Lam JS, Berghuis AM, Creuzenet C. (2005)
- Towards a better understanding of the substrate specificity of the UDP-N-acetylglucosamine C4 epimerase WbpP. Biochem J. 389: 173-80.
- Bernatchez S, Szymanski CM, Ishiyama N, Li J, Jarrell HC, Lau PC, Berghuis AM, Young NM, Wakarchuk WW. (2005)
- A single bifunctional UDP-GlcNAc/Glc 4-epimerase supports the synthesis of three cell surface glycoconjugates in Campylobacter jejuni. J Biol Chem. 280: 4792-802.