High throughput microarray platform: Validation and use in characterizing kinase

Paul S. Kayne, PhD, Applied Genomics, Bristol-Myers Squibb Co. As gene expression profiling experiments become more complex, new tools are needed for successful prosecution. Improvements needed include larger batch sizes, higher throughput, increased reproducibility and new methods of analysis. Here we present a validation of a new high throughput array system. Through automation and new designs, this system enables large scale experimental designs. Understanding the mechanisms and effects of compounds is an area that has largely been underserved by gene expression profiling, due in part to modestly designed experiments. We will discuss our approach to understanding kinase inhibitors. We employ the high throughput arrays to undertake designs incorporating dose curves, and new methods for analysis. Taken together, we are able to better understand the actions of these inhibitors on multiple targets of action.