Translation of in vitro results into in vivo applications

Unlike the ideal in vitro conditions in which major guidelines for drug efficacy are routinely established in the laboratory under static conditions, it is natural that high variability arises in the clinical situation, which is intrinsically dynamic, and thus brings up concerns about the applicability of principles established in vitro. Founding of a theoretical framework for the information transfer from in vitro to in vivo conditions is crucial for the design of clinical studies. When the experimental results are ready to be transferred into clinical practice, the intrinsic variability has to be accounted for by moving from the static description to more developed dynamic and stochastic versions. Hence, there is a need for novel mechanistic-based studies to be undertaken which will increase the explanatory power for the interaction of the organism with drugs in a therapeutic assessment ( Atherton, Nekka).